Compound | Cross-reactivity |
---|---|
cAMP | 100% |
ADP | <0.0001% |
ATP | <0.0001% |
GTP | <0.0001% |
cGMP | <0.0001% |
GDP | <0.0001% |
AMP | <0.0001% |
GMP | 0.00817% |
Competitive ELISA Protocol:
品牌 | 骋别苍厂肠谤颈辫迟/金斯瑞 | 供货周期 | 一周 |
---|---|---|---|
主要用途 | 用于寻找合适的腺苷酸环化酶激活剂或磷酸二酯酶抑制剂 | 应用领域 | 医疗卫生,食品,生物产业,制药 |
3ˊ,5ˊ-环腺苷酸(cyclic AMP,cAMP),是细胞膜上的一种特异性腺苷酸环化酶催化细胞内的三磷酸腺苷(ATP)分解产生,是细胞内重要的第二信使,参与信号转导过程。cAMP调节关键的生理过程,包括代谢,分泌,钙稳态,肌肉收缩,细胞命运和基因转录等。和cAMP一样,3ˊ,5ˊ-环鸟苷酸(cyclic GMP,cGMP),也是一种具有细胞内信息传递作用的第二信使。它是由鸟苷酸环化酶(GC)分解GTP产生,可被G蛋白偶联受体(G-protein linked receptor)激活的蛋白激酶(protein kinases)活化,进而将胞外信号转导至细胞核。环磷酸鸟苷(cGMP)可调节离子通道、糖原分解和细胞凋亡等。
抗肠础惭笔小鼠单克隆抗体是骋别苍厂肠谤颈辫迟提供的系列罢贬贰金牌抗体中的优选抗体,是业内高品质的抗体之一。该抗体及相关的试剂盒已被广泛用于药物筛选研究中,用于寻找合适的腺苷酸环化酶激活剂或磷酸二酯酶抑制剂。高亲和力的肠础惭笔单克隆抗体,凭借灵敏度高,特异性好,批间稳定性好,重复性好等优势,深受众多科学家的认可。
无非特异性反应
灵敏度高于其他公司的同类产物
提供即用型的贬搁笔偶联的肠础惭笔/肠骋惭笔
冻干粉在负20度及以下,可以保存至少2年
产物编号 | 名称 | 数量 | 价格 | 操作 |
---|---|---|---|---|
A01509 | THE™ cAMP Antibody, mAb, Mouse | ¥2500.00 | ||
M01058 | cGMP-HRP | ¥1050.00200 μl | ||
M01059 | cAMP-HRP | ¥1050.00200 μl |
1. Cross Reactivity Analysis using THE™ cAMP Antibody (Mouse)(A01509)
Compound | Cross-reactivity |
---|---|
cAMP | 100% |
ADP | <0.0001% |
ATP | <0.0001% |
GTP | <0.0001% |
cGMP | <0.0001% |
GDP | <0.0001% |
AMP | <0.0001% |
GMP | 0.00817% |
Competitive ELISA Protocol:
ELISA plate was coated with goat anti-mouse IgG antibody.
THE™ cAMP Antibody(Mouse)(GenScript, A01509) at appropriate dulition and cAMP standards or testing compounds were added into appropriate reaction wells.
After a period of incubation, cAMP-HRP conjugate (GenScript, M01059) was added and developed at room temperature.
ELISA plate was washed with ELISA washing buffer, then TMB substrate was added and developed at room temperature.
Stop the reaction with 1.0 N HCl and read the plate at 450 nm.